ResQ Biotech has developed the specialized Escherichia coli strains SuptoxD and SuptoxR for high-level production of difficult-to-express integral membrane proteins.
When used as expression hosts, these strains exert a dual positive effect on recombinant membrane protein production compared to wild-type bacteria:
(1) they suppress the toxicity that frequently accompanies the membrane protein overexpression process, thus enabling enhanced levels of final bacterial biomass, and
(2) they markedly increase the cellular accumulation of membrane-incorporated and properly folded protein.
Combined, these two positive effects result in dramatically enhanced volumetric yields for various recombinant membrane proteins of both prokaryotic and eukaryotic origin.